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mouse anti e2f4  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse anti e2f4
    Mouse Anti E2f4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 288 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+e2f4/E2F-4+Antibody/pm36427391-61-68-72
    Average 93 stars, based on 288 article reviews
    mouse anti e2f4 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Loss of Rbl2 (Retinoblastoma‐Like 2) Exacerbates Myocardial Ischemia/Reperfusion Injury
    Article Snippet: (glyceraldehyde- 3- phosphate dehydrogenase, 5174, Cell Signaling Technology, 1:1000). .. Immunofluorescence staining was performed with the following primary antibodies: rabbit anti- phospho- Rbl2 (S952, ab68126, Abcam, 1:100), mouse anti- cardiac Troponin T (MS- 295- P, ThermoFisher Scientific, 1:100), rabbit anti- phospho- CDK2 (Thr160, 2561, Cell Signaling Technology, 1:50), mouse anti- E2F1 (sc- 251, Santa Cruz Biotechnology, 1:100), mouse anti- E2F4 (sc- 511, Santa Cruz Biotechnology, 1:100), and rabbit anti- sarcomeric α- actinin (ab68167, Abcam, 1:100). .. Apoptosis was assessed by terminal deoxynucleotidyl transferase- mediated 2′- deoxyuridine 5′- triphosphate nick- end labeling (TUNEL) staining using the in situ Cell Death Detection Kit (Roche Applied Science) according to the manufacturer’s instructions.

    Staining:

    Article Title: Loss of Rbl2 (Retinoblastoma‐Like 2) Exacerbates Myocardial Ischemia/Reperfusion Injury
    Article Snippet: (glyceraldehyde- 3- phosphate dehydrogenase, 5174, Cell Signaling Technology, 1:1000). .. Immunofluorescence staining was performed with the following primary antibodies: rabbit anti- phospho- Rbl2 (S952, ab68126, Abcam, 1:100), mouse anti- cardiac Troponin T (MS- 295- P, ThermoFisher Scientific, 1:100), rabbit anti- phospho- CDK2 (Thr160, 2561, Cell Signaling Technology, 1:50), mouse anti- E2F1 (sc- 251, Santa Cruz Biotechnology, 1:100), mouse anti- E2F4 (sc- 511, Santa Cruz Biotechnology, 1:100), and rabbit anti- sarcomeric α- actinin (ab68167, Abcam, 1:100). .. Apoptosis was assessed by terminal deoxynucleotidyl transferase- mediated 2′- deoxyuridine 5′- triphosphate nick- end labeling (TUNEL) staining using the in situ Cell Death Detection Kit (Roche Applied Science) according to the manufacturer’s instructions.

    Article Title: Cigarette smoke-induced cell cycle arrest in spermatocytes [GC-2spd(ts)] is mediated through crosstalk between Ahr-Nrf2 pathway and MAPK signaling.
    Article Snippet: .. The cells stained with mouse anti-E2F4 (Santa Cruz; dilution 1:50) for 30 min at RT were washed twice in blocking buffer, and incubated in Alexa Fluor 488-conjugated goat anti-mouse secondary antibody (dilution 1:2000) along with the nuclear dye, TO-PRO-3 iodide (Molecular Probes) for 30 min at RT. .. Mouse immunoglobulin G1 (IgG2a) (Sigma-Aldrich) isotype control at equal concentration served as negative control.

    Blocking Assay:

    Article Title: Cigarette smoke-induced cell cycle arrest in spermatocytes [GC-2spd(ts)] is mediated through crosstalk between Ahr-Nrf2 pathway and MAPK signaling.
    Article Snippet: .. The cells stained with mouse anti-E2F4 (Santa Cruz; dilution 1:50) for 30 min at RT were washed twice in blocking buffer, and incubated in Alexa Fluor 488-conjugated goat anti-mouse secondary antibody (dilution 1:2000) along with the nuclear dye, TO-PRO-3 iodide (Molecular Probes) for 30 min at RT. .. Mouse immunoglobulin G1 (IgG2a) (Sigma-Aldrich) isotype control at equal concentration served as negative control.

    Incubation:

    Article Title: Cigarette smoke-induced cell cycle arrest in spermatocytes [GC-2spd(ts)] is mediated through crosstalk between Ahr-Nrf2 pathway and MAPK signaling.
    Article Snippet: .. The cells stained with mouse anti-E2F4 (Santa Cruz; dilution 1:50) for 30 min at RT were washed twice in blocking buffer, and incubated in Alexa Fluor 488-conjugated goat anti-mouse secondary antibody (dilution 1:2000) along with the nuclear dye, TO-PRO-3 iodide (Molecular Probes) for 30 min at RT. .. Mouse immunoglobulin G1 (IgG2a) (Sigma-Aldrich) isotype control at equal concentration served as negative control.

    Article Title: E2F4 regulates cell cycle to mediate embryonic development in pigs.
    Article Snippet: In mammals, E2 factor (E2F) acts as a cell cycle regulator.. E2F transcription factor 4 (E2F4) is a member of the E2F family of transcription factors and usually represents predominant E2F activity in cells.. The E2F4 gene has been extensively studied in animals and is associated with multiple functions, such as cell cycle regulation and apoptosis; however, little is known about its role during embryonic development.

    other:

    Article Title: PAF remodels the DREAM complex to bypass cell quiescence and promote lung tumorigenesis
    Article Snippet: Mouse anti-E2F4 , Santa Cruz Biotechnology , Cat# sc-398543.

    Western Blot:

    Article Title: Loss of Rbl2 (Retinoblastoma‐Like 2) Exacerbates Myocardial Ischemia/Reperfusion Injury
    Article Snippet: Cells and heart tissue were homogenized in radioimmune precipitation assay buffer supplemented with protease and phosphatase inhibitors (ThermoFisher Scientific). .. Western blotting was performed with the following antibodies: rabbit anti- phospho- Rbl2 (S952, ab68136, Abcam, 1:1000), mouse anti- Rbl2 (610 262, BD Biosciences, 1:1000), rabbit anti- phospho- CDK2 (cyclin- dependent kinase 2) (Thr160, 2561, Cell Signaling Technology, 1:1000), rabbit anti- CDK2 (sc163, Santa Cruz Biotechnology, 1:1000), rabbit antipoly (ADP- ribose) polymerase (9542, Cell Signaling Technology, 1:1000), rabbit anti- caspase 3 (9662, Cell Signaling Technology, 1:1000), rabbit anti- cleaved caspase 3 (9664s, Cell Signaling Technology, 1:500), rabbit anti- Bnip3 (3769, Cell Signaling Technology, 1:1000), mouse anti- E2F1 (sc- 251, Santa Cruz Biotechnology, 1:1000), mouse anti- E2F4 (sc- 511, Santa Cruz Biotechnology, 1:1000), and rabbit anti- GAPDH ..

    Synthesized:

    Article Title: Characterization of the mechanism by which the RB/E2F pathway controls expression of the cancer genomic DNA deaminase APOBEC3B
    Article Snippet: .. Antibodies were rabbit anti-RAD51, 1:10,000 (Abcam, ab133534), mouse anti-E2F4, 1:250 (Santa Cruz, sc-398543), rabbit anti-E2F6, 1:500 (Abcam, ab53061), rabbit anti-HA, 1:5000 (Cell Signaling, C29F4), mouse anti-Rb, 1:300 (Santa Cruz, sc-102), mouse anti-E2F1, 1:1000 (Santa Cruz, sc-251), mouse anti-E2F3, 1:800 (Santa Cruz, sc-56665), mouse anti-E2F5, 1:800 (Santa Cruz, sc-374268), mouse anti-E2F6, 1:300 (Santa Cruz, sc-53273), mouse anti-LIN9, 1:300 (Santa Cruz, sc-398234) mouse anti-tubulin, 1:20,000 (Sigma-Aldrich, T5168), and rabbit anti-A3B, 1:1,000 [5210-87-13] ( ). mRNA quantification: mRNA was extracted (GenElute, Sigma-Aldrich) and cDNA was synthesized using SuperScript First-Strand RT (ThermoFisher). mRNA expression of all APOBEC3 family members and TBP was quantified by RT-qPCR with specific primers ( ) in Ssofast Supermix (Bio-Rad) using a Lightcycler (Roche). ..

    Expressing:

    Article Title: Characterization of the mechanism by which the RB/E2F pathway controls expression of the cancer genomic DNA deaminase APOBEC3B
    Article Snippet: .. Antibodies were rabbit anti-RAD51, 1:10,000 (Abcam, ab133534), mouse anti-E2F4, 1:250 (Santa Cruz, sc-398543), rabbit anti-E2F6, 1:500 (Abcam, ab53061), rabbit anti-HA, 1:5000 (Cell Signaling, C29F4), mouse anti-Rb, 1:300 (Santa Cruz, sc-102), mouse anti-E2F1, 1:1000 (Santa Cruz, sc-251), mouse anti-E2F3, 1:800 (Santa Cruz, sc-56665), mouse anti-E2F5, 1:800 (Santa Cruz, sc-374268), mouse anti-E2F6, 1:300 (Santa Cruz, sc-53273), mouse anti-LIN9, 1:300 (Santa Cruz, sc-398234) mouse anti-tubulin, 1:20,000 (Sigma-Aldrich, T5168), and rabbit anti-A3B, 1:1,000 [5210-87-13] ( ). mRNA quantification: mRNA was extracted (GenElute, Sigma-Aldrich) and cDNA was synthesized using SuperScript First-Strand RT (ThermoFisher). mRNA expression of all APOBEC3 family members and TBP was quantified by RT-qPCR with specific primers ( ) in Ssofast Supermix (Bio-Rad) using a Lightcycler (Roche). ..

    Quantitative RT-PCR:

    Article Title: Characterization of the mechanism by which the RB/E2F pathway controls expression of the cancer genomic DNA deaminase APOBEC3B
    Article Snippet: .. Antibodies were rabbit anti-RAD51, 1:10,000 (Abcam, ab133534), mouse anti-E2F4, 1:250 (Santa Cruz, sc-398543), rabbit anti-E2F6, 1:500 (Abcam, ab53061), rabbit anti-HA, 1:5000 (Cell Signaling, C29F4), mouse anti-Rb, 1:300 (Santa Cruz, sc-102), mouse anti-E2F1, 1:1000 (Santa Cruz, sc-251), mouse anti-E2F3, 1:800 (Santa Cruz, sc-56665), mouse anti-E2F5, 1:800 (Santa Cruz, sc-374268), mouse anti-E2F6, 1:300 (Santa Cruz, sc-53273), mouse anti-LIN9, 1:300 (Santa Cruz, sc-398234) mouse anti-tubulin, 1:20,000 (Sigma-Aldrich, T5168), and rabbit anti-A3B, 1:1,000 [5210-87-13] ( ). mRNA quantification: mRNA was extracted (GenElute, Sigma-Aldrich) and cDNA was synthesized using SuperScript First-Strand RT (ThermoFisher). mRNA expression of all APOBEC3 family members and TBP was quantified by RT-qPCR with specific primers ( ) in Ssofast Supermix (Bio-Rad) using a Lightcycler (Roche). ..

    Chromatin Immunoprecipitation:

    Article Title: Characterization of the mechanism by which the RB/E2F pathway controls expression of the cancer genomic DNA deaminase APOBEC3B
    Article Snippet: .. ChIP antibodies were mouse anti-E2F4 (Santa Cruz, sc-398543), rabbit anti-E2F6 (Abcam, ab53061), and rabbit anti-L3MBTL2 (Active Motif, 39569). ..



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    Image Search Results


    Reagents and tools table

    Journal: The EMBO Journal

    Article Title: p53 regulates DREAM complex-mediated repression in a p21-independent manner

    doi: 10.1038/s44318-025-00402-7

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: Mouse anti-E2F4 (used for WB, ChIP, reChIP, reChIP seq) , Millipore , Cat# 05-312, RRID: AB_2097110 (WB: 1:2500, ChIP: 2 μg/reaction, reChIP: 3 μg/reaction, reChIP seq: 4 μg/reaction).

    Techniques: Reverse Transcription, SYBR Green Assay, Extraction, Bicinchoninic Acid Protein Assay, Plasmid Preparation, Mutagenesis, Recombinant, Cloning, Expressing, Control, Sequencing, Software